E2 Rapid Quantitative Test (Chemiluminescence Immunoassay)

Application

Estradiol (E2), a potent estrogen, controls various biological processes by functioning as a ligand for the nuclear receptors estrogen receptor alpha (ERα) and estrogen receptor beta (ERβ). These estrogen receptors (ERs) can participate in both the genomic (transcriptional) and non-genomic actions of E2.

The genomic action of E2 involves the binding of ERα to the regulatory regions of target genes, either directly or through protein-protein interactions. Once DNA-bound, ERα recruits various co-regulatory molecules to induce chromatin modifications that can either increase or decrease the level of target gene transcription.

Several extracellular signal-activated kinases can also phosphorylate and modulate the activity of ERα. This phosphorylation-mediated modulation of ERα activity may be responsible for altered E2 responses, such as resistance to anti-estrogen therapy in breast cancer.

The major kinases that have been found to modulate ERα activity include:

1. ERK1/2 (Extracellular signal-regulated Kinases 1 and 2)

2. AKT (also known as Protein Kinase B)

3. RSK (Ribosomal S6 Kinase)

4. PAK1 (p21-Activated Kinase 1)

5. p38 kinase

6. SRC (Proto-Oncogene Tyrosine-Protein Kinase Src)

The complex interplay between the genomic and non-genomic actions of E2, mediated through the estrogen receptors and various signaling kinases, highlights the multifaceted mechanisms by which estradiol controls diverse physiological processes.

Product Specification

Features

Smart - Photosensitive and Luminescent Microspheres

Rapid - Whole Blood Sample Type Supported & No Centrifuge Needed

Convenient - Room Temperature Storage & Minimal sample consumption

Clinical significance

Monitoring Ovarian Function and Menstrual Cycle

Assessment of Ovulation

Evaluation of Menopausal Status

Monitoring Hormone Replacement Therapy (HRT)

Evaluation of Gynecological Disorders